your example of the 6 iron from the rough would be the same as off the tee....
the stronger player may be able to deliver more speed at impact from the rough - so the parameters would not be the same.....but if 3 different players (weak, normal, strong) delivered 80mph, the ball would go the same distance
But grass and dirt (rough) certainly offer more resistance than air (tee ball) to a golf club. Therefore an accelerating object would be slowed down less by greater resistance, than a decelerating object. Real world analogy - when your foot is on the gas and you need to break quickly, it's a lot harder to stop than when your already slowing down.
So the scientist in me (not a physicist, just a B.Sc. Chemistry), suggests there would be a difference.
How significant a difference there is, is the big question, I'd love to see some properly conducted experimental data on this but I haven't seen any.
Remember it could be insignificant. Or not. Most of the scientists favoured by Brian seem to suggest it's insignificant, but I'm not sure if there talking of about the simplified tee balled case, without factoring grass/ground resistances.
Maybe a question for the conference?
p.s. When double checking my classical physics 101 equations for Kinetic energy and Force - I came across this:
the kinetic energy equation by Miles Mathis
Curious what our physicists make of this paper?